Analysis Note

Positive ControlRat or mouse liver cytosol

Application

ELISA (direct only) (1:2000-1:4000)r>Immunoblotting (1:500-1:4000)r>Immunocytochemistry (1:200-1:1000)r>Immunoprecipitation (not recommended)

General description

This Anti-IDE, N-Terminal (97-273) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of IDE, N-Terminal (97-273).

Recognizes the ~115 kDa endogenous and recombinant IDE.

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~115 kDa endogenous as well as recombinant IDE protein.

Immunogen

a recombinant protein consisting of amino acids 97-273 of rat IDE fused to GST

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Morelli, L., et al. 2003. J. Biol. Chem.278, 23221.Kurochkin, I.V. and Goto, S. 1994. FEBS Lett.345, 33.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)